Diarrhetic Shellfish Poisoning

Rapid test for diarrhetic shellfish poisoning (DSP) from Neogen® can screen shellfish samples at 160 ppb.

Reveal® 2.0 for DSP is a single step immunochromatographic lateral flow assay used for the rapid and practical qualitative analysis of shellfish possibly contaminated by OA group toxins (OA, DTX1, DTX2 and DTX3). These marine biotoxins can be accumulated in shellfish during toxic algal blooms. Once the toxin is present in the shellfish tissue it cannot be destroyed by heat during cooking. The US and EU have established maximum threshold levels of 160 ppb. The test can detect as little as 160 ppb of OA toxins in shellfish samples.

Neogen Reveal 2.0 test systems have everything to complete a pathogen screen, including the enrichment medium. Reveal’s lateral flow device combines an immunoassay with chromatography for a rapid and accurate one-step result. Built-in visual control lines provide definitive interpretations.


Reveal® 2.0 for DSP

Reveal® 2.0 for DSP (diarrhetic shellfish poisoning) is a single step lateral flow assay that detects okadaic acid (OA) and dinophysistoxin (DTX) derivatives. These marine biotoxins can accumulate in shellfish during toxic algal blooms. Once the toxin is present in the shellfish tissue it cannot be destroyed by heat during cooking. The E.U. and U.S. have established maximum threshold levels of 160 ppb.

Features Benefits & Product Specifications

Result

Reference Materials

  • 9561 - Reveal 2.0 for DSP (Diarrhetic Shellfish Poisoning), Material Safety Data Sheet [English]
  • 24 Reveal 2.0 for DSP lateral flow test strips
  • 24 wells
  • 24 vials of DSP buffer A (clear cap)
  • 24 vials of DSP buffer B (red cap)
  • 25 extraction bags
  • 48 disposable 100 µL pipettors
  • Marine biotoxins starter kit (Neogen® item 9563)
    • Microwell holder
    • 1 roller
    • 1 bag clip (white clip and green straw)
  • DSP Hydrolysis Pack (Neogen item 9554)
    • 2.5 M sodium hydroxide (NaOH) solution (5 mL)
    • 2.5 M hydrochloric acid (HCl) solution (5 mL)
    • 24 glass sample vials
  • Distilled water
  • Methanol (analytical grade recommended, VWR 20864.320)
  • Filter syringes (Neogen item 9420)
  • Sample collection cups with lids (Neogen items 9428, 9428B)
  • Blender (Neogen items 9493, 9477 or 9495)
  • Scale capable of weighing 0.5–400 g ± 0.1 g (Neogen item 9427)
  • Timer (Neogen item 9452)
  • Graduated cylinder, 50 mL (Neogen item 9447)
  • Sample collection tubes with caps, 5 mL (Neogen item 9421, 9421B)
  • Pipettor, 100–1000 mL, and pipette tips (Neogen item 9463, 9464)
  • Heater block (capable of holding 76 ± 2°C (Recommended VWR 460-3250 & 460-3280)
  • AccuScan Pro reader (Neogen item 9565)
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