Veratox® for Gliadin R5

Veratox® for Gliadin R5/Gluten is used for the quantitative analysis of ingredients, clean-in-place rinses and finished food products intended to be gluten free for the presence of gliadin and prolamins found in wheat, barley and rye

Features Benefits & Product Specifications

Features and Benefits

Gliadin R5

Veratox® for Gliadin R5

  • Accurate and easy-to-use
  • Cost effective microwell format
  • High specificity
Product Specifications

Gliadin R5

Veratox® for Gliadin R5

  • Range of quantification: 2.5 – 40 ppm
  • Controls provided:
    • 0, 2.5, 5, 10, 20 and 40 ppm gliadin
    • 0, 10, 20, 40 and 100 ppm gluten
  • Testing time: 30 minutes
  • Tests per kit: Up to 38
  • Approvals: AOAC-RI 061201

Result

Reference Materials

  • 8432S - Allergen Environmental Swabbing Kit (100 swabs and tips), Material Safety Data Sheet [English]
  • 8510 - Veratox for Gliadin R5, Procedures [English]
  • 8510 - Veratox for Gliadin R5, Safety Data Sheet [English]
  • 8515 - Gliadin Renaturing Cocktail Solution, 100 mL, Safety Data Sheet [English]
  • 8515B - Gliadin Renaturing Cocktail Solution, 500 mL, Safety Data Sheet [English]
  • 8515S - Gliadin Renaturing Cocktail Solution, 25 mL, Safety Data Sheet [English]
  • 48 antibody-coated microwells
  • 48 red-marked transfer wells
  • 6 yellow-labeled bottles of 0, 2.5, 5, 10, 20, and 40 ppm gliadin controls (0, 5, 10, 20, 40, and 80 ng/mL gliadin)
  • 2 blue-labeled bottles of enzyme-labeled antibody conjugate
  • 1 green-labeled bottle of K-Blue® Substrate
  • 1 red-labeled bottle of Red Stop solution
  • 40 mL of 10 mM PBS-Tween washing reagent in a widemouth bottle. Each bottle can prepare 1 L in distilled or deionized water (pH 7.4)
  • 1 foil pouch sample diluent concentrate of 10 mM PBS dry powder containing enough powder to prepare 1 L of dilution buffer
  • 50 g of extraction additive in 2 specimen cups
  • Plastic scoop to measure extraction additive
  • Microwell Reader with 650 nm filter (Neogen® item 9303)
  • Graduated cylinder 10-20 mL (Neogen item 9447)
  • Graduated cylinder 250 mL (Neogen item 9368)
  • 1-Liter bottle (Neogen item 9472)
  • Pipettor, 50-200 µL adjustable (Neogen item 9276)
  • Pipettor, 12-channel 50-200µL (Neogen item 9273)
  • Pipette tips (Neogen item 9410, 9407, 9417)
  • Wash bottle (Neogen item 9400)
  • Well holder (Neogen item 9402)
  • Timer (Neogen item 9426)
  • Reagent boats, set of 3 (Neogen item 9435)
  • 7 mL tube or vial (Neogen item 9468)
  • Pipette pump for serological tubes (Neogen item 9277)
  • Pipettes, sterile serological, 10 mL (pack of 100) (Neogen item 9415)
  • Laboratory grade ethanol (≥190 proof)
  • Distilled water
  • Waterproof marker
  • Paper towel or equivalent
  • Special extraction additive for dark chocolate, cocoa and tannin (Neogen item 8482)
  • Centrifuge (optional)
  • Vortex (optional)
    • Non-heat treated method (extraction A or B)
    • Tube, 50 mL (Neogen item 9381) or bottle, 125 mL (Neogen item 9398)
    • Scale ± 0.1 g (Neogen item 9427, 9395)
    • Orbital rotator (Neogen item 9396) or shaker to hold 50 mL tube
    Heat-treated cocktail method (extraction C)
    • Gliadin renaturing cocktail solution for heat processed samples (Neogen item 8515, 8515B, 8515S)
    • Orbital rotator (Neogen item 9396)
    • Scale, ± 0.01g (Neogen Item 9395)
    • Water bath or oven at 50°C

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