The Orange Serum Broth (OSB) vial detects aciduric microorganism contamination in high acid food products. When carbon dioxide (CO2) is released by growing yeasts and molds, the CO2 produced diffuses through a membrane layer into a soft agar plug containing a dye indicator. The color change in the dye is read by the Soleris® instrument. The membrane layer also serves as a barrier, eliminating product interference with the reading frame.