Yeast Acid Food Vial is a minimally buffered medium with dextrose as the carbon source, and chlorophenol red (CPR) dye as the pH indicator (pH range: 6.0 to 6.4). A supplement containing chloramphenicol and chlortetracycline is used to inhibit the growth of bacteria. As the yeast grow and reduce the sugar to acid, the CPR dye changes from red to yellow.