- 8480 - Veratox for Gliadin, Safety Data Sheet [English]
- 8482 - Gliadin Extraction Additive for Dark Chocolate, Cocoa and Tannin, Safety Data Sheet [English]
- 8483 - Gliadin Test Cocktail Solution, Heat Processed Samples, 100 mL, Safety Data Sheet [English]
- 8483B - Gliadin Test Cocktail Solution, Heat Processed Samples, 500 mL, Material Safety Data Sheet [English]
- 8515 - Gliadin Renaturing Cocktail Solution, 100 mL, Safety Data Sheet [English]
- 8515B - Gliadin Renaturing Cocktail Solution, 500 mL, Safety Data Sheet [English]
- 8515S - Gliadin Renaturing Cocktail Solution, 25 mL, Safety Data Sheet [English]
- 48 antibody-coated microwells
- 48 red-marked transfer wells
- 5 yellow-labeled bottles of 0, 5, 10, 20, and 50 ppm gliadin (0, 10, 20, 40 and 100 ppm gluten) controls (0, 12.5, 25, 50, and 125 ng/mL gliadin)
- 2 blue-labeled bottles of enzyme-labeled antibody conjugate
- 1 green-labeled bottle of K-Blue® Substrate
- 1 red-labeled bottle of Red Stop solution
- 40 mL of 10 mM PBS-Tween washing reagent in a wide mouth bottle. Each bottle can prepare 1 L in distilled or deionized water (pH 7.4)
- 1 foil pouch of 10 mM PBS dry powder containing enough powder to prepare 1 L of dilution solvent
- 50 g of extraction additive in 2 specimen cups
- Plastic scoop to measure extraction additive
Universal materials for all extraction and test methods
- Microwell Reader with 650 nm filter (Neogen® item 9303)
- Graduated cylinder 10-20 mL (Neogen item 9447)
- Graduated cylinder 250 mL (Neogen item 9368)
- 1-Liter bottle (Neogen item 9472)
- Pipettor, 50-200 µL adjustable (Neogen item 9276)
- Pipettor, 12-channel 50-200µL (Neogen item 9273)
- Pipette tips (Neogen item 9410, 9407, 9417)
- Wash bottle (Neogen item 9400)
- Well holder (Neogen item 9402)
- Timer (Neogen item 9426)
- Reagent boats, set of 3 (Neogen item 9435)
- 7 mL tube or vial (Neogen item 9468)
- Pipette pump for serological tubes (Neogen item 9277)
- Pipettes, sterile serological, 10 mL (pack of 100) (Neogen item 9415)
- Laboratory grade ethanol (≥190 proof)
- Distilled water
- Waterproof marker
- Paper towel or equivalent
- Special extraction additive for dark chocolate, cocoa and tannin (Neogen item 8482)
- Centrifuge (optional)
- Vortex (optional)
Non-heat treated method (extraction A or B)
- Tube, 50 mL (Neogen item 9381) or bottle, 125 mL (Neogen item 9398)
- Scale ± 0.1 g (Neogen item 9427, 9395)
- Orbital rotator (Neogen item 9396) or shaker to hold 50 mL tube
Heat-treated cocktail method (extraction C)
- Gliadin renaturing cocktail solution for heat processed samples (Neogen item 8515, 8515B, 8515S)
- Orbital rotator (Neogen item 9396)
- Scale, ± 0.01g (Neogen Item 9395)
- Water bath or oven at 50°C
