Veratox® for Histamine

Veratox® for Histamine is a competitive direct ELISA intended for the quantitative analysis of histamine in scombroid species of fish, such as tuna, bluefish and mahi-mahi, and in fishmeal.

Features Benefits & Product Specifications

Features and Benefits

Histamine

Veratox® for Histamine

  • Simple water extraction, no chemicals necessary
  • Equivalent recovery to traditional fluorometric methods
  • Quantitative test 1 or up to 19 samples at one time
Product Specifications

Histamine

Veratox® for Histamine

  • Lower limit of detection: 2 ppm
  • Range of quantitation: 2.5–40 ppm
  • Controls provided: 0, 2.5, 5, 10, 20 and 50 ppm
  • Testing time: 20 minutes
  • Antibody cross-reactivity: Specific for histamine
  • Tests per kit: Up to 38
  • Approvals: AOAC-RI 070703

Result

Reference Materials

  • 9505 - Veratox for Histamine, Procedures [English]
  • 9505 - Veratox for Histamine for lot #266708 and newer, Safety Data Sheet [English]
  • 9505 - Veratox for Histamine, Safety Data Sheet [English]
  • 48 antibody-coated microwells
  • 48 red-marked mixing wells
  • 6 yellow-labeled bottles of 0, 2.5, 5, 10, 20 and 50 ppm histamine controls
  • 1 blue-labeled bottle of histamine-HRP conjugate solution
  • 1 foil pouch of sample extract diluent buffer concentrate of 10 mM PBS dry powder
  • 1 bottle of 40 mL wash buffer concentrate of 10 mM PBS-Tween
  • 1 green-labeled bottle of K-Blue® Substrate solution
  • 1 red-labeled bottle of Red Stop solution
  • Veratox for Histamine Extraction Kit (Neogen® item 9510)—Contains 38 bottles, 38 filter syringes and 38 tubes
  • Disposable bottles with 125 mL capacity (Neogen item 9398)
  • Filter syringes for histamine (Neogen item 9240H), Whatman #1 filter paper (Neogen item 9340), centrifuge or equivalent
  • Sample collection tubes (Neogen items 9421, 9421B)
  • 100 mL graduated cylinder
  • Blender
  • Test tube rack (Neogen item 9440)
  • Tubes, 15 mL, graduated (Neogen item 9474)
  • Scale capable of weighing 10–50 grams (Neogen item 9427)
  • Microwell strip reader with a 650 nm filter (Neogen item 9303)
  • Pipettor, 12-channel (Neogen item 9273)
  • Pipettor, 100 µL (Neogen item 9272)
  • Tips for 12-channel and 100 µL pipettors (Neogen items 9410, 9407, 9417)
  • Paper towels or equivalent absorbent material
  • Microwell holder (Neogen item 9402)
  • Timer (Neogen item 9426)
  • Waterproof marker
  • Wash bottle (Neogen item 9400)
  • 1 L bottle with lid (Neogen item 9472)
  • 2 reagent boats for 12-channel pipettor (Neogen item 9435)
  • Distilled or deionized water

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