Veratox® brand of diagnostics from Neogen® are quantitative microwell enzyme-linked immunosorbent assay (ELISA) tests. By running multiple controls and establishing a standard curve through a reader, samples can be plotted against the curve to determine the concentration of analyte present.

Veratox diagnostics can be either sandwich or direct competitive, they may also be read at a 650 nm or 450 nm wavelength, depending on the specifics of the kit. These kits are often viewed as the gold standard by the food industry, particularly in allergen and mycotoxin testing.

Each test kit contains antibody-coated microwells with antibodies specific to the kit’s target substance.

First, samples and controls are added to their respective test wells. Next, an enzyme conjugate (target substance chemically linked with an enzyme) is added. The samples/controls and conjugate are mixed and transferred to antibody wells where they compete for the antibody binding sites. The more target substance in the sample, the less conjugate that binds in the wells.

After an incubation, the wells are washed to remove all unbound materials.

A substrate, which changes color in the presence of the conjugate, is added to the wells. During an incubation, blue color develops in proportion to the amount of conjugate versus target substance in the wells. The more conjugate bound, the more blue color that develops, indicating less substance present.

Results are obtained by measuring the wells’ color change in a microwell reader and comparing the readings against a standard curve.

Veratox® MAX brand of diagnostics from Neogen® are quantitative microwell enzyme-linked immunosorbent assay (ELISA) tests. By running multiple controls and establishing a standard curve through a reader, samples can be plotted against the curve to determine the concentration of analyte present.

The Veratox MAX format utilizes an aqueous extraction procedure eliminating the cost and hazard of solvents in your laboratory.